Hotspot

ycf4-cemA

LSC · 60831-61721 · 891 bp

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Pi 0.0145
MarkerSeek score 66.00
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
891
variable_sites
44
pi
0.0145
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 GAGCCATTCCATTGACTCGT GAGAAATCCACCAGGGCAAA 50.0 50.0 58.0 58.1 1059-1094 bp 1.0 85.8
ycf4-cemA_p2 CTCCACGGGAAATGGAACAA GAGAAATCCACCAGGGCAAA 50.0 50.0 58.1 58.1 1023-1058 bp 1.0 85.5
ycf4-cemA_p3 GAGCCATTCCATTGACTCGT AGAAATCCACCAGGGCAAAA 50.0 45.0 58.0 57.6 1058-1093 bp 1.0 83.2
ycf4-cemA_p4 CTCCACGGGAAATGGAACAA AGAAATCCACCAGGGCAAAA 50.0 45.0 58.1 57.6 1022-1057 bp 1.0 82.9
ycf4-cemA_p5 GGAGCCATTCCATTGACTCG GAGAAATCCACCAGGGCAAA 55.0 50.0 58.7 58.1 1060-1095 bp 1.0 80.5