Hotspot

ndhC-trnV_uac

LSC · 50653-51544 · 892 bp

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Pi 0.0142
MarkerSeek score 70.50
Primer available yes
Label ndhC-trnV_uac

Feature Metadata

label_name
ndhC-trnV_uac
feature_type
igs
region
LSC
length_bp
892
variable_sites
48
pi
0.0142
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.90
markerseek_score
70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV_uac_p1 GTCCTTTGCTAATCGGAGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 905-1037 bp 1.0 83.8
ndhC-trnV_uac_p2 CGGTCCTTTGCTAATCGGAG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.4 57.8 907-1039 bp 1.0 82.9
ndhC-trnV_uac_p3 CTCCGGTCCTTTGCTAATCG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.4 57.8 910-1042 bp 1.0 82.9
ndhC-trnV_uac_p4 GTCCTTTGCTAATCGGAGCT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.7 57.5 904-1036 bp 1.0 81.1
ndhC-trnV_uac_p5 GGTCCTTTGCTAATCGGAGC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.7 57.8 906-1038 bp 1.0 80.7