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trnT_ugu-trnL_uaa

LSC · 46621-47368 · 748 bp

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Pi 0.0344
MarkerSeek score 70.80
Primer available yes
Label trnT_ugu-trnL_uaa

Feature Metadata

label_name
trnT_ugu-trnL_uaa
feature_type
igs
region
LSC
length_bp
748
variable_sites
67
pi
0.0344
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.88
markerseek_score
70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT_ugu-trnL_uaa_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 674-809 bp 1.0 88.2
trnT_ugu-trnL_uaa_p2 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 706-841 bp 1.0 87.0
trnT_ugu-trnL_uaa_p3 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 668-803 bp 1.0 87.0
trnT_ugu-trnL_uaa_p4 GGAATCGAACCGATGACCAT CGTCTACCGATTTCGCCATA 50.0 50.0 57.8 57.6 700-835 bp 1.0 85.8
trnT_ugu-trnL_uaa_p5 GCGATGCTCTAACCTCTGAG CGTAGCGTCTACCGATTTCG 55.0 55.0 57.9 58.6 673-808 bp 1.0 85.3