Hotspot

psbC-trnS_uga

LSC · 35034-35262 · 229 bp

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Pi 0.019
MarkerSeek score 69.20
Primer available yes
Label psbC-trnS_uga

Feature Metadata

label_name
psbC-trnS_uga
feature_type
igs
region
LSC
length_bp
229
variable_sites
18
pi
0.019
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS_uga_p1 CTGTTCTTTCCATGACCCCC GAGAGATGGCTGAGTGGTTG 55.0 55.0 58.2 58.0 334-355 bp 1.0 84.9
psbC-trnS_uga_p2 TCTTCGTAGGGCATTTGTGG AAGTCCCGGTCTTGAAAACC 50.0 50.0 57.9 58.0 397-418 bp 1.0 84.3
psbC-trnS_uga_p3 TCTTCGTAGGGCATTTGTGG GAGAGATGGCTGAGTGGTTG 50.0 55.0 57.9 58.0 418-439 bp 1.0 84.1
psbC-trnS_uga_p4 TCTTCGTAGGGCATTTGTGG TGGCTGAGTGGTTGAAAGTC 50.0 50.0 57.9 58.0 412-433 bp 1.0 84.0
psbC-trnS_uga_p5 TCTTCGTAGGGCATTTGTGG GTCTTTCGGAGAGATGGCTG 50.0 55.0 57.9 58.1 426-447 bp 1.0 83.6