Hotspot

trnR_ucu-trnG_ucc

LSC · 29783-30042 · 260 bp

Back to Aster

Pi 0.0154
MarkerSeek score 60.40
Primer available yes
Label trnR_ucu-trnG_ucc

Feature Metadata

label_name
trnR_ucu-trnG_ucc
feature_type
igs
region
LSC
length_bp
260
variable_sites
13
pi
0.0154
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.92
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR_ucu-trnG_ucc_p1 AGAAGACCTCTGTCCTATCCA AGCCTTCCAAGCTAACGATG 47.6 50.0 56.9 58.0 309-340 bp 1.0 70.5
trnR_ucu-trnG_ucc_p2 AGAAGACCTCTGTCCTATCCA CCTAGCCTTCCAAGCTAACG 47.6 55.0 56.9 58.1 312-343 bp 1.0 70.3
trnR_ucu-trnG_ucc_p3 AGGTTTAGAAGACCTCTGTCCT AGCCTTCCAAGCTAACGATG 45.5 50.0 57.9 58.0 315-346 bp 1.0 69.8
trnR_ucu-trnG_ucc_p4 AGGTTTAGAAGACCTCTGTCCT CCTAGCCTTCCAAGCTAACG 45.5 55.0 57.9 58.1 318-349 bp 1.0 69.6
trnR_ucu-trnG_ucc_p5 AGAAGACCTCTGTCCTATCCA CCCTAGCCTTCCAAGCTAAC 47.6 55.0 56.9 57.7 313-344 bp 1.0 68.1