Hotspot

trnK_uuu-rps16

LSC · 4227-5043 · 817 bp

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Pi 0.00927
MarkerSeek score 62.90
Primer available yes
Label trnK_uuu-rps16

Feature Metadata

label_name
trnK_uuu-rps16
feature_type
igs
region
LSC
length_bp
817
variable_sites
28
pi
0.00927
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK_uuu-rps16_p1 TCCTAGCCGCACTTAAAAGC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.3 58.3 947-1005 bp 1.0 81.7
trnK_uuu-rps16_p2 CCGCACTTAAAAGCCGAGTA TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.3 58.3 941-999 bp 1.0 81.6
trnK_uuu-rps16_p3 TCGCGGTCTTCCAAACTTTA TTCCTTGAAAAAGGCGCTCA 45.0 45.0 57.5 58.3 1013-1071 bp 1.0 79.4
trnK_uuu-rps16_p4 CGCGGTCTTCCAAACTTTAC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.4 58.3 1012-1070 bp 1.0 79.0
trnK_uuu-rps16_p5 TCCTAGCCGCACTTAAAAGC TCCTTGAAAAAGGCGCTCAG 50.0 50.0 58.3 58.8 946-1004 bp 1.0 78.1