Hotspot

ndhF

SSC · 113307-115511 · 2205 bp

Back to Astelia

Pi 0.0132

MarkerSeek score 50.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2205
variable_sites: 29
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAGACCCTTCCCTTTGTTCG	AGAATTGGTGAATCGGAAACA	50.0	38.1	57.7	55.8	2465-2636 bp	1.0	53.8
ndhF_p2	AATGCCGTCGCCTATTGTTA	AGAATTGGTGAATCGGAAACA	45.0	38.1	57.7	55.8	2605-2776 bp	1.0	53.4
ndhF_p3	ATGCCGTCGCCTATTGTTAC	AGAATTGGTGAATCGGAAACA	50.0	38.1	58.4	55.8	2604-2775 bp	1.0	52.5
ndhF_p4	TACCGAAGAAGACCCTTCCC	AGAATTGGTGAATCGGAAACA	55.0	38.1	58.4	55.8	2473-2644 bp	1.0	52.5
ndhF_p5	ACCAAGTTCAACGTTAGCCA	AGAATTGGTGAATCGGAAACA	45.0	38.1	57.7	55.8	2863 bp	0.5	31.6