Hotspot

trnH-rpl2

IRb · 86930-86972 · 43 bp

Back to Astelia

Pi 0.116

MarkerSeek score 74.40

Primer available yes

Label trnH-rpl2

Feature Metadata

label_name: trnH-rpl2
feature_type: igs
region: IRb
length_bp: 43
variable_sites: 5
pi: 0.116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-rpl2_p1	TGGATCAAGGCAGTGGATTG	TTCTTCGTCGCCGTAAATAG	50.0	45.0	57.9	55.7	122 bp	1.0	64.0
trnH-rpl2_p2	GGATCAAGGCAGTGGATTGT	TTCTTCGTCGCCGTAAATAG	50.0	45.0	57.9	55.7	121 bp	1.0	64.0
trnH-rpl2_p3	GTGGATCAAGGCAGTGGATT	TTCTTCGTCGCCGTAAATAG	50.0	45.0	57.9	55.7	123 bp	1.0	63.9
trnH-rpl2_p4	CGGATGTAGCCAAGTGGATC	TTCTTCGTCGCCGTAAATAG	55.0	45.0	58.1	55.7	136 bp	1.0	63.7
trnH-rpl2_p5	GATTGTGAATCCACCATGCG	TTCTTCGTCGCCGTAAATAG	50.0	45.0	57.5	55.7	107 bp	1.0	61.7