Hotspot

rps8-rpl14

LSC · 83074-83275 · 202 bp

Back to Astelia

Pi 0.0248

MarkerSeek score 66.70

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 202
variable_sites: 5
pi: 0.0248
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AACCGTTCCGTTTTTACCCA	GAACTCGAGTTTTTGGTGCG	45.0	50.0	58.0	58.0	360-367 bp	1.0	80.6
rps8-rpl14_p2	AACCGTTCCGTTTTTACCCA	GCTCGGGAATTGAGACAGTT	45.0	50.0	58.0	57.9	337-344 bp	1.0	80.0
rps8-rpl14_p3	AACCGTTCCGTTTTTACCCA	ACTCGAGTTTTTGGTGCGAT	45.0	45.0	58.0	58.1	358-365 bp	1.0	79.7
rps8-rpl14_p4	ACCGTTCCGTTTTTACCCAT	GAACTCGAGTTTTTGGTGCG	45.0	50.0	57.7	58.0	359-366 bp	1.0	78.8
rps8-rpl14_p5	CGAACCGTTCCGTTTTTACC	GAACTCGAGTTTTTGGTGCG	50.0	50.0	57.7	58.0	362-369 bp	1.0	78.6