Hotspot

psbE-petL

LSC · 67028-68363 · 1336 bp

Back to Astelia

Pi 0.0169

MarkerSeek score 71.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1336
variable_sites: 20
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACGAATAACCAACCTGCA	AAGCAGCCAGTAGAAAACCG	45.0	50.0	57.4	58.5	1344-1494 bp	1.0	76.9
psbE-petL_p2	TGACGAATAACCAACCTGCA	GCAGCCAGTAGAAAACCGAA	45.0	50.0	57.4	58.5	1342-1492 bp	1.0	76.9
psbE-petL_p3	CAAAAGAACGTTCTCCCGTG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.3	58.5	1260-1410 bp	1.0	76.1
psbE-petL_p4	CAAAAGAACGTTCTCCCGTG	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.3	58.5	1258-1408 bp	1.0	76.0
psbE-petL_p5	CAGCAAAAGAACGTTCTCCC	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.3	58.5	1263-1413 bp	1.0	75.9