Hotspot

ndhC-trnV

LSC · 52287-53946 · 1660 bp

Back to Astelia

Pi 0.0226

MarkerSeek score 68.10

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 1660
variable_sites: 37
pi: 0.0226
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	CGGGGCTAAAACTCCAGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1775-1792 bp	1.0	79.6
ndhC-trnV_p2	ATCGGGGCTAAAACTCCAGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	1777-1794 bp	1.0	77.8
ndhC-trnV_p3	AATCGGGGCTAAAACTCCAG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.6	57.8	1778-1795 bp	1.0	77.8
ndhC-trnV_p4	TCCTTCACTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	1787-1804 bp	1.0	77.5
ndhC-trnV_p5	CGGGGCTAAAACTCCAGAAA	AGCCCAATGCAACAAAATGC	50.0	45.0	57.8	58.5	1857-1874 bp	1.0	77.3