Hotspot

trnR-atpA

LSC · 10685-10803 · 119 bp

Back to Astelia

Pi 0.0254

MarkerSeek score 66.60

Primer available yes

Label trnR-atpA

Feature Metadata

label_name: trnR-atpA
feature_type: igs
region: LSC
length_bp: 119
variable_sites: 3
pi: 0.0254
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-atpA_p1	GGAATGAAAAGCGTCCATTGT	TGCTACCAAGACATTCACCG	42.9	50.0	57.4	57.9	294-295 bp	1.0	68.3
trnR-atpA_p2	GGTTCAAATCCTATTGGACGC	TGCTACCAAGACATTCACCG	47.6	50.0	57.3	57.9	234-235 bp	1.0	67.1
trnR-atpA_p3	GGAATGAAAAGCGTCCATTGT	GCTACCAAGACATTCACCGA	42.9	50.0	57.4	57.6	293-294 bp	1.0	66.0
trnR-atpA_p4	GGAATGAAAAGCGTCCATTGT	TACCAAGACATTCACCGAGC	42.9	50.0	57.4	57.6	291-292 bp	1.0	66.0
trnR-atpA_p5	AGGAATGAAAAGCGTCCATTG	TGCTACCAAGACATTCACCG	42.9	50.0	57.1	57.9	296 bp	0.5	44.4