Hotspot

trnK-rps16

LSC · 4055-4751 · 697 bp

Back to Astelia

Pi 0.0253

MarkerSeek score 74.70

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 697
variable_sites: 17
pi: 0.0253
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	797-811 bp	1.0	82.6
trnK-rps16_p2	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	796-810 bp	1.0	82.6
trnK-rps16_p3	AAAGCCGAGTACTCTACCGT	CTTGAAAAAGGCGCTCAACC	50.0	50.0	58.2	58.2	801-815 bp	1.0	81.0
trnK-rps16_p4	AAGCCGAGTACTCTACCGTT	CCTTGAAAAAGGCGCTCAAC	50.0	50.0	58.2	58.2	801-815 bp	1.0	81.0
trnK-rps16_p5	AAGCCGAGTACTCTACCGTT	CTTGAAAAAGGCGCTCAACC	50.0	50.0	58.2	58.2	800-814 bp	1.0	81.0