Hotspot

ndhF

Genome · 105936-108173 · 2238 bp

Back to Asplenium

Pi 0.0829

MarkerSeek score 79.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2238
variable_sites: 550
pi: 0.0829
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GTCGGGAGAAATGAACACGA	CCGCTGTATTGCCATCTTCT	50.0	50.0	57.9	58.0	2744-2907 bp	0.666667	77.7
ndhF_p2	AGTCGGGAGAAATGAACACG	CCGCTGTATTGCCATCTTCT	50.0	50.0	57.9	58.0	2745-2908 bp	0.666667	77.7
ndhF_p3	AGTAGCAGTTTACGGCAAGG	CCGCTGTATTGCCATCTTCT	50.0	50.0	57.9	58.0	2703-2866 bp	0.666667	77.6
ndhF_p4	GTCGGGAGAAATGAACACGA	TCCGCTGTATTGCCATCTTC	50.0	50.0	57.9	58.0	2745-2908 bp	0.5	71.0
ndhF_p5	AGTCGGGAGAAATGAACACG	TCCGCTGTATTGCCATCTTC	50.0	50.0	57.9	58.0	2746-2909 bp	0.5	71.0