Hotspot

ndhB

Genome · 1-1183 · 1183 bp

Back to Asplenium

Pi 0.0866

MarkerSeek score 81.40

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: Genome
length_bp: 1183
variable_sites: 295
pi: 0.0866
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	ACGAAGGATCAAGCTCACTA	GAAGCAGAGAGAGGCAAGTC	45.0	55.0	56.0	58.0	229-240 bp	0.25	45.6
ndhB_p2	TACGAAGGATCAAGCTCACT	GAAGCAGAGAGAGGCAAGTC	45.0	55.0	56.0	58.0	230-241 bp	0.25	45.6
ndhB_p3	ACGAAGGATCAAGCTCACTA	GAAATCAACTCTTGCCGTGC	45.0	50.0	56.0	58.0	288-298 bp	0.25	43.8
ndhB_p4	ACGAAGGATCAAGCTCACTA	ACTAGGGCAATGGAAGGTCT	45.0	50.0	56.0	58.0	939-949 bp	0.25	40.5
ndhB_p5	TACGAAGGATCAAGCTCACT	ACTAGGGCAATGGAAGGTCT	45.0	50.0	56.0	58.0	940-950 bp	0.25	40.5