Hotspot

ccsA-ndhD

Genome · 111427-112276 · 850 bp

Back to Asplenium

Pi 0.158

MarkerSeek score 68.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: Genome
length_bp: 850
variable_sites: 179
pi: 0.158
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.41
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AATGGGTTGTCATGTGCAGT	TTTAGTGCATTCTCCCTCGC	45.0	50.0	58.0	58.0	1385-1894 bp	0.5	71.8
ccsA-ndhD_p2	ATGGGTTGTCATGTGCAGTT	TTTAGTGCATTCTCCCTCGC	45.0	50.0	58.0	58.0	1384-1893 bp	0.5	71.8
ccsA-ndhD_p3	GAGCTGGGACCCAAAAGAAA	TTTAGTGCATTCTCCCTCGC	50.0	50.0	58.0	58.0	921-1430 bp	0.5	71.8
ccsA-ndhD_p4	ATGGGTTGTCATGTGCAGTT	AGGATATGGGCTGATTCGGA	45.0	50.0	58.0	58.0	1745-2254 bp	0.5	71.8
ccsA-ndhD_p5	TCCACCTACCATCCCCAAAT	TTTAGTGCATTCTCCCTCGC	50.0	50.0	58.0	58.0	1085-1581 bp	0.166667	53.4