Hotspot

rrn16-rps12

Genome · 93445-94657 · 1213 bp

Back to Asplenium

Pi 0.051

MarkerSeek score 86.50

Primer available yes

Label rrn16-rps12

Feature Metadata

label_name: rrn16-rps12
feature_type: igs
region: Genome
length_bp: 1213
variable_sites: 222
pi: 0.051
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-rps12_p1	TCCGACTTGCATGTGTTAGG	GAACTCTCGCGACTTTACGT	50.0	50.0	57.9	58.0	1148-1346 bp	0.833333	84.3
rrn16-rps12_p2	TCCGACTTGCATGTGTTAGG	CTCTCGCGACTTTACGTAGG	50.0	55.0	57.9	57.9	1145-1343 bp	0.833333	83.9
rrn16-rps12_p3	TCCGACTTGCATGTGTTAGG	AGCCGTTACCTCAAACTTGG	50.0	50.0	57.9	58.1	1177-1375 bp	0.833333	83.7
rrn16-rps12_p4	TCCGACTTGCATGTGTTAGG	GCCGTTACCTCAAACTTGGA	50.0	50.0	57.9	58.1	1176-1374 bp	0.833333	83.7
rrn16-rps12_p5	TCCGACTTGCATGTGTTAGG	GAGGTTAGTCGAACTCTCGC	50.0	55.0	57.9	57.8	1158-1356 bp	0.833333	83.2