Hotspot

rpl20-rps12

Genome · 65661-66654 · 994 bp

Back to Asplenium

Pi 0.0757

MarkerSeek score 82.90

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: Genome
length_bp: 994
variable_sites: 240
pi: 0.0757
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCTCGTTTTACTCTGGTCA	CAAAATCTCCCGCTCTTCGA	45.0	50.0	55.8	58.0	1057-1115 bp	0.916667	70.4
rpl20-rps12_p2	TCCTCGTTTTACTCTGGTCA	GGACAAAATCTCCCGCTCTT	45.0	50.0	55.8	57.9	1060-1118 bp	0.916667	69.5
rpl20-rps12_p3	TCCTCGTTTTACTCTGGTCA	ACAAAATCTCCCGCTCTTCG	45.0	50.0	55.8	58.3	1058-1116 bp	0.916667	68.2
rpl20-rps12_p4	TCCTCGTTTTACTCTGGTCA	AACGGAGAGGAGTGTGTACT	45.0	50.0	55.8	57.7	1027-1085 bp	0.916667	68.2
rpl20-rps12_p5	TCCTCGTTTTACTCTGGTCA	AAAATCTCCCGCTCTTCGAG	45.0	50.0	55.8	57.7	1056-1114 bp	0.833333	64.6