Hotspot

trnW-CCA-trnP-UGG

Genome · 63708-63868 · 161 bp

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Pi 0.121

MarkerSeek score 80.90

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: Genome
length_bp: 161
variable_sites: 59
pi: 0.121
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	CACGCCCTGTAGGATTTGAA	GGTAGCGTGTTTGTTTTGGG	50.0	50.0	57.9	57.9	278-300 bp	1.0	90.1
trnW-CCA-trnP-UGG_p2	ACGCCCTGTAGGATTTGAAC	GGTAGCGTGTTTGTTTTGGG	50.0	50.0	57.9	57.9	277-299 bp	1.0	90.1
trnW-CCA-trnP-UGG_p3	AGTAGTAAGCACGCCCTGTA	GGTAGCGTGTTTGTTTTGGG	50.0	50.0	58.2	57.9	287-309 bp	1.0	89.7
trnW-CCA-trnP-UGG_p4	GTAGTAAGCACGCCCTGTAG	GGTAGCGTGTTTGTTTTGGG	55.0	50.0	57.8	57.9	286-308 bp	1.0	89.3
trnW-CCA-trnP-UGG_p5	CACGCCCTGTAGGATTTGAA	CGGTAGCGTGTTTGTTTTGG	50.0	50.0	57.9	58.3	279-301 bp	1.0	89.1