Hotspot

psbE-petL

Genome · 62234-63020 · 787 bp

Back to Asplenium

Pi 0.0914

MarkerSeek score 81.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 787
variable_sites: 216
pi: 0.0914
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TTTGGATTCGGTTGTGTCGT	TTCAAATCCTACAGGGCGTG	45.0	50.0	58.0	57.9	1841-1885 bp	0.833333	82.9
psbE-petL_p2	TTTGGATTCGGTTGTGTCGT	GTTCAAATCCTACAGGGCGT	45.0	50.0	58.0	57.9	1842-1886 bp	0.833333	82.9
psbE-petL_p3	AAACGGGGAGGGTATGGTAA	TTCAAATCCTACAGGGCGTG	50.0	50.0	58.0	57.9	1512-1530 bp	0.5	68.3
psbE-petL_p4	AAACGGGGAGGGTATGGTAA	GTTCAAATCCTACAGGGCGT	50.0	50.0	58.0	57.9	1513-1531 bp	0.5	68.3
psbE-petL_p5	AAACGGGGAGGGTATGGTAA	TGAAATAGTAGGCCCCGTCA	50.0	50.0	58.0	58.1	1044 bp	0.083333	42.0