Hotspot

petA-psbJ

Genome · 58899-61467 · 2569 bp

Back to Asplenium

Pi 0.131

MarkerSeek score 67.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 2569
variable_sites: 290
pi: 0.131
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.25
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGGGAAAGGGTGGATACGAA	GCCGGTACACTTGTGATAGG	50.0	55.0	58.0	58.1	911-2949 bp	0.75	81.2
petA-psbJ_p2	AGGGAAAGGGTGGATACGAA	CTGTGGTTGGTAGGTACTGC	50.0	55.0	58.0	57.9	932-2970 bp	0.75	80.9
petA-psbJ_p3	AGGGAAAGGGTGGATACGAA	GTGGTTGGTAGGTACTGCAG	50.0	55.0	58.0	57.9	930-2968 bp	0.75	80.9
petA-psbJ_p4	TGATCAGCCGCTGACTAGTA	GCCGGTACACTTGTGATAGG	50.0	55.0	57.9	58.1	1087-2834 bp	0.25	58.8
petA-psbJ_p5	GTTGTACTCCAAGACCCGTC	GCCGGTACACTTGTGATAGG	55.0	55.0	57.9	58.1	1045-2776 bp	0.166667	52.7