Hotspot

ndhC-trnM-CAU

Genome · 47864-48992 · 1129 bp

Back to Asplenium

Pi 0.132

MarkerSeek score 82.20

Primer available yes

Label ndhC-trnM-CAU

Feature Metadata

label_name: ndhC-trnM-CAU
feature_type: igs
region: Genome
length_bp: 1129
variable_sites: 417
pi: 0.132
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM-CAU_p1	TCATATTGGTGTAGCAGAAACA	TTCGAACCAATGACTCTCGC	36.4	50.0	55.5	58.3	1118-1221 bp	1.0	53.3
ndhC-trnM-CAU_p2	TCATATTGGTGTAGCAGAAACA	CAATGACTCTCGCCGTATGA	36.4	50.0	55.5	57.5	1111-1214 bp	1.0	52.0
ndhC-trnM-CAU_p3	TCATATTGGTGTAGCAGAAACA	CCAATGACTCTCGCCGTATG	36.4	55.0	55.5	58.5	1112-1215 bp	1.0	52.0
ndhC-trnM-CAU_p4	TCATATTGGTGTAGCAGAAACA	TGACTCTCGCCGTATGAAAG	36.4	50.0	55.5	57.4	1108-1211 bp	1.0	52.0
ndhC-trnM-CAU_p5	TCATATTGGTGTAGCAGAAACAT	TTCGAACCAATGACTCTCGC	34.8	50.0	56.0	58.3	1118-1221 bp	1.0	52.0