Hotspot

psbK-psbI

Genome · 7153-7743 · 591 bp

Back to Asplenium

Pi 0.106

MarkerSeek score 81.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: Genome
length_bp: 591
variable_sites: 195
pi: 0.106
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GGGGAAAAGTCTTGCACTGA	GAATCCCTCTCTCTCCCCTC	50.0	60.0	58.0	58.0	1526 bp	0.083333	43.1
psbK-psbI_p2	GGGGAAAAGTCTTGCACTGA	ACTTGGTTTTGAGCGGAACT	50.0	45.0	58.0	57.9	2053 bp	0.083333	42.8
psbK-psbI_p3	GGGGAAAAGTCTTGCACTGA	GAGAGATGGCTGAGAGGTCT	50.0	55.0	58.0	57.9	1596 bp	0.083333	42.6
psbK-psbI_p4	GGGGAAAAGTCTTGCACTGA	TTCGAATCCCTCTCTCTCCC	50.0	55.0	58.0	57.9	1529 bp	0.083333	42.6
psbK-psbI_p5	GATTCGAACCTCCGAGTGAC	GAATCCCTCTCTCTCCCCTC	55.0	60.0	58.1	58.0	1376 bp	0.083333	42.5