Hotspot

matK-rps16

Genome · 2900-3545 · 646 bp

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Pi 0.111

MarkerSeek score 81.80

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: Genome
length_bp: 646
variable_sites: 206
pi: 0.111
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	GTCGACGAATTAAGCGGACT	CTGTGTGCACTCACCTATCC	50.0	55.0	58.1	58.0	1396-1439 bp	0.75	78.6
matK-rps16_p2	TCGACGAATTAAGCGGACTG	CTGTGTGCACTCACCTATCC	50.0	55.0	58.1	58.0	1395-1438 bp	0.75	78.6
matK-rps16_p3	GTCGACGAATTAAGCGGACT	CCTGTGTGCACTCACCTATC	50.0	55.0	58.1	58.0	1397-1440 bp	0.666667	75.3
matK-rps16_p4	CGACGAATTAAGCGGACTGA	CTGTGTGCACTCACCTATCC	50.0	55.0	58.1	58.0	1399-1437 bp	0.583333	72.0
matK-rps16_p5	CGACGAATTAAGCGGACTGA	CCTGTGTGCACTCACCTATC	50.0	55.0	58.1	58.0	1400-1438 bp	0.5	68.2