Hotspot

rps11

LSC · 83947-84369 · 423 bp

Back to Aspidopterys

Pi 0.011

MarkerSeek score 48.10

Primer available yes

Label rps11

Feature Metadata

label_name: rps11
feature_type: gene
region: LSC
length_bp: 423
variable_sites: 9
pi: 0.011
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11_p1	AAGCTCTTCGCATCGCAATA	GCAAAGGGGGATTGGATTCT	45.0	50.0	58.1	57.8	767 bp	0.25	48.2
rps11_p2	ATGCGTCACGAGTTCCATAC	GCAAAGGGGGATTGGATTCT	50.0	50.0	58.1	57.8	929 bp	0.25	48.1
rps11_p3	AAGCTCTTCGCATCGCAATA	GGTAATCTCCGGGACTTCCT	45.0	55.0	58.1	58.2	1354 bp	0.25	47.8
rps11_p4	ATGCGTCACGAGTTCCATAC	GGTAATCTCCGGGACTTCCT	50.0	55.0	58.1	58.2	1516 bp	0.25	47.7
rps11_p5	TACGATAACCACGATTCCGC	GCAAAGGGGGATTGGATTCT	50.0	50.0	57.9	57.8	1085 bp	0.25	47.7