Hotspot

trnR-ACG-rrn5

IRa · 136369-136609 · 241 bp

Back to Aspidopterys

Pi 0.0207

MarkerSeek score 38.30

Primer available yes

Label trnR-ACG-rrn5

Feature Metadata

label_name: trnR-ACG-rrn5
feature_type: igs
region: IRa
length_bp: 241
variable_sites: 10
pi: 0.0207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-rrn5_p1	ACTTGCTCTGCTATTCTGCC	CCTTGTTCCTACATGACCCG	50.0	55.0	58.0	58.0	913-918 bp	1.0	82.1
trnR-ACG-rrn5_p2	ACTTGCTCTGCTATTCTGCC	GGCATCACAATCACTAGCCA	50.0	50.0	58.0	58.0	753-758 bp	1.0	82.1
trnR-ACG-rrn5_p3	ACTTGCTCTGCTATTCTGCC	AGTCATGGGGCATCACAATC	50.0	50.0	58.0	57.9	761-766 bp	1.0	81.9
trnR-ACG-rrn5_p4	ACTTGCTCTGCTATTCTGCC	GTCAAGTGGAAGTGCAGTGA	50.0	50.0	58.0	58.0	974-979 bp	1.0	81.7
trnR-ACG-rrn5_p5	ACTTGCTCTGCTATTCTGCC	CTCTGCCCTCCCTCTCTATC	50.0	60.0	58.0	58.1	823-828 bp	0.75	69.1