Hotspot

rps11-rpl36

LSC · 84370-84488 · 119 bp

Back to Aspidopterys

Pi 0.0921

MarkerSeek score 76.50

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 119
variable_sites: 19
pi: 0.0921
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	ACGTCCATTCGTACTCTTGC	GGCGGAATGGGGATTGTAAT	50.0	50.0	58.0	58.0	716-861 bp	1.0	87.9
rps11-rpl36_p2	AAGACCAAGAGATCACCCGA	GGCGGAATGGGGATTGTAAT	50.0	50.0	58.1	58.0	834-979 bp	1.0	87.1
rps11-rpl36_p3	ACCGTCCGAATAGCATTTCC	GGCGGAATGGGGATTGTAAT	50.0	50.0	58.1	58.0	919-1064 bp	1.0	87.0
rps11-rpl36_p4	AGGGGTTACATCTCGGACAA	GGCGGAATGGGGATTGTAAT	50.0	50.0	58.1	58.0	1043-1188 bp	1.0	86.7
rps11-rpl36_p5	AAGCTCTTCGCATCGCAATA	GGCGGAATGGGGATTGTAAT	45.0	50.0	58.1	58.0	1325-1463 bp	1.0	86.2