Hotspot

psbE-petL

LSC · 68992-70397 · 1406 bp

Back to Aspidopterys

Pi 0.00624

MarkerSeek score 64.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1406
variable_sites: 17
pi: 0.00624
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1494-1521 bp	1.0	73.9
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCA	55.0	50.0	58.2	57.1	1489-1516 bp	1.0	73.5
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	GTCGTATCTTGCTCAGACCA	50.0	50.0	58.4	57.1	1487-1514 bp	1.0	72.3
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCAA	50.0	47.6	57.8	57.8	1494-1521 bp	1.0	71.5
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGCTCAGACC	50.0	50.0	57.8	56.8	1495-1522 bp	1.0	71.5