Hotspot

petA-psbJ

LSC · 67123-68201 · 1079 bp

Back to Aspidopterys

Pi 0.0182
MarkerSeek score 64.50
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1079
variable_sites
33
pi
0.0182
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGAGAAGGTTCAATTGTCCGA ATTCCGGATTGGGTTCATCC 42.9 50.0 57.2 57.6 1117-1139 bp 1.0 67.1
petA-psbJ_p2 TGAGAAGGTTCAATTGTCCGAA ATTCCGGATTGGGTTCATCC 40.9 50.0 57.9 57.6 1117-1139 bp 1.0 64.5
petA-psbJ_p3 TTGAGAAGGTTCAATTGTCCGA ATTCCGGATTGGGTTCATCC 40.9 50.0 57.9 57.6 1118-1140 bp 1.0 64.5
petA-psbJ_p4 TGAGAAGGTTCAATTGTCCG ATTCCGGATTGGGTTCATCC 45.0 50.0 55.7 57.6 1117-1139 bp 1.0 62.7
petA-psbJ_p5 TTGAGAAGGTTCAATTGTCCG ATTCCGGATTGGGTTCATCC 42.9 50.0 56.5 57.6 1118-1140 bp 1.0 60.9