Hotspot

rbcL-accD

LSC · 59982-60972 · 991 bp

Back to Aspidopterys

Pi 0.014

MarkerSeek score 67.70

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 991
variable_sites: 25
pi: 0.014
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CCGTGAAGCTAGCAAATGGA	AGAATCGGGTCCTCACTGAA	50.0	50.0	58.3	58.1	1108-1155 bp	1.0	81.8
rbcL-accD_p2	TCCGTGAAGCTAGCAAATGG	AGAATCGGGTCCTCACTGAA	50.0	50.0	58.3	58.1	1109-1156 bp	1.0	81.8
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	AGAATCGGGTCCTCACTGAA	55.0	50.0	57.7	58.1	1090-1137 bp	1.0	81.6
rbcL-accD_p4	CCGTGAAGCTAGCAAATGGA	ATCGGGTCCTCACTGAAACT	50.0	50.0	58.3	58.4	1105-1152 bp	1.0	79.4
rbcL-accD_p5	TCCGTGAAGCTAGCAAATGG	ATCGGGTCCTCACTGAAACT	50.0	50.0	58.3	58.4	1106-1153 bp	1.0	79.4