Hotspot

ndhC-trnV-UAC

LSC · 53582-54782 · 1201 bp

Back to Aspidopterys

Pi 0.0181
MarkerSeek score 64.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1201
variable_sites
38
pi
0.0181
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCCAGAAAATATCATATTCGT GTTCGAGTCCGTATAGCCCT 34.8 55.0 56.1 58.7 1225-1258 bp 1.0 46.5
ndhC-trnV-UAC_p2 TGCCCAGAAAATATCATATTCGT AGAAGGTCTACGGTTCGAGT 34.8 50.0 56.1 57.8 1237-1270 bp 1.0 46.4
ndhC-trnV-UAC_p3 TGCCCAGAAAATATCATATTCGT GAAGGTCTACGGTTCGAGTC 34.8 55.0 56.1 57.5 1236-1269 bp 1.0 46.4
ndhC-trnV-UAC_p4 ATGCCCAGAAAATATCATATTCGT AGAAGGTCTACGGTTCGAGT 33.3 50.0 56.6 57.8 1238-1271 bp 1.0 46.4
ndhC-trnV-UAC_p5 TGCCCAGAAAATATCATATTCGT AAGGTCTACGGTTCGAGTCC 34.8 55.0 56.1 58.8 1235-1268 bp 1.0 46.4