Hotspot

petN-psbM

LSC · 30072-31538 · 1467 bp

Back to Aspidopterys

Pi 0.00592

MarkerSeek score 63.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1467
variable_sites: 16
pi: 0.00592
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCACTGTTCATTCTAGTCCC	50.0	47.6	58.2	57.7	1497-1623 bp	1.0	72.9
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1503-1629 bp	1.0	72.3
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGCACTGTTCATTCTAGTCCC	50.0	47.6	57.6	57.7	1436-1562 bp	1.0	71.4
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1442-1568 bp	1.0	70.8
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	GCACTGTTCATTCTAGTCCCT	50.0	47.6	58.2	57.4	1496-1622 bp	1.0	70.7