Hotspot

trnK-UUU-rps16

LSC · 4378-5236 · 859 bp

Back to Aspidopterys

Pi 0.0169
MarkerSeek score 68.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
859
variable_sites
25
pi
0.0169
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.2 58.0 963-988 bp 1.0 83.7
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.2 58.0 962-987 bp 1.0 83.7
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT CCACAGGAACCGTTCATGAT 50.0 50.0 58.2 57.9 940-965 bp 1.0 83.0
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT CCACAGGAACCGTTCATGAT 50.0 50.0 58.2 57.9 939-964 bp 1.0 83.0
trnK-UUU-rps16_p5 GTTAGCCGCACTTAAAAGCC TTTCCTTGAAAGGGGTGCTC 50.0 50.0 57.7 58.0 977-1002 bp 1.0 82.9