Hotspot

petD

LSC · 77757-78999 · 1243 bp

Back to Aspidistra

Pi 0.0029

MarkerSeek score 58.40

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1243
variable_sites: 30
pi: 0.0029
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ACTTCTCTTCAACTCCGAAGT	CCCTAGATACCTACACGTGGT	42.9	52.4	56.6	58.1	1334-1362 bp	0.9375	53.8
petD_p2	ACTTCTCTTCAACTCCGAAGT	GCACACGATCCATATTTTCGC	42.9	47.6	56.6	58.4	1414-1442 bp	0.96875	52.8
petD_p3	ACTTCTCTTCAACTCCGAAGT	TCCCTAGATACCTACACGTGG	42.9	52.4	56.6	57.8	1335-1363 bp	0.9375	52.5
petD_p4	TGGATACTTCTCTTCAACTCCG	CCCTAGATACCTACACGTGGT	45.5	52.4	57.3	58.1	1339-1367 bp	0.9375	51.1
petD_p5	AGTTGAAGTGGATTCTCGAAGA	CCCTAGATACCTACACGTGGT	40.9	52.4	57.1	58.1	1288-1316 bp	0.9375	50.0