Hotspot

ndhD

SSC · 117112-118614 · 1503 bp

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Pi 0.00389

MarkerSeek score 44.00

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 43
pi: 0.00389
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGGGACCCCAAAGAAACTTG	ACCGACAAAAACCCGTACTC	50.0	50.0	57.9	58.1	2018-2022 bp	1.0	81.2
ndhD_p2	TGGGACCCCAAAGAAACTTG	TGATACCTTGGGACGGATGT	50.0	50.0	57.9	58.1	2224-2228 bp	1.0	81.1
ndhD_p3	GAGCAGTATGGGCTAACGAG	ACCGACAAAAACCCGTACTC	55.0	50.0	57.9	58.1	2059-2063 bp	1.0	80.7
ndhD_p4	GAGCAGTATGGGCTAACGAG	TGATACCTTGGGACGGATGT	55.0	50.0	57.9	58.1	2265-2269 bp	1.0	80.6
ndhD_p5	TCTGGAGCAGTATGGGCTAA	TGATACCTTGGGACGGATGT	50.0	50.0	57.8	58.1	2269-2273 bp	1.0	80.3