Hotspot

rps12-trnV-GAC

IRb · 100101-101980 · 1880 bp

Back to Aspidistra

Pi 0.00156

MarkerSeek score 57.80

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: IRb
length_bp: 1880
variable_sites: 18
pi: 0.00156
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	TGTGGCCAATACCAGGGATA	GATGACTTCCACCACGTCAA	50.0	50.0	58.1	57.8	1950-2034 bp	1.0	82.6
rps12-trnV-GAC_p2	TGTGGCCAATACCAGGGATA	AACTGATGACTTCCACCACG	50.0	50.0	58.1	57.8	1954-2038 bp	1.0	82.6
rps12-trnV-GAC_p3	TGTGGCCAATACCAGGGATA	ATGACTTCCACCACGTCAAG	50.0	50.0	58.1	57.8	1949-2033 bp	1.0	82.6
rps12-trnV-GAC_p4	AATTGTGGCCAATACCAGGG	GATGACTTCCACCACGTCAA	50.0	50.0	58.1	57.8	1953-2037 bp	1.0	82.3
rps12-trnV-GAC_p5	AATTGTGGCCAATACCAGGG	ATGACTTCCACCACGTCAAG	50.0	50.0	58.1	57.8	1952-2036 bp	1.0	82.3