Hotspot

rpl33-rps18

LSC · 69068-69279 · 212 bp

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Pi 0.0139

MarkerSeek score 58.10

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 212
variable_sites: 14
pi: 0.0139
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	GCACAATACACCCAGTCGAT	CAATTCGATCCCCCGATTCA	50.0	50.0	58.0	57.7	353-382 bp	1.0	85.7
rpl33-rps18_p2	GCACAATACACCCAGTCGAT	TCGATCCCCCGATTCAATTG	50.0	50.0	58.0	57.7	349-378 bp	1.0	85.7
rpl33-rps18_p3	AGAATCGGCACAATACACCC	CAATTCGATCCCCCGATTCA	50.0	50.0	58.0	57.7	360-389 bp	1.0	85.5
rpl33-rps18_p4	AGAATCGGCACAATACACCC	TCGATCCCCCGATTCAATTG	50.0	50.0	58.0	57.7	356-385 bp	1.0	85.5
rpl33-rps18_p5	AATCGGCACAATACACCCAG	TCGATCCCCCGATTCAATTG	50.0	50.0	58.2	57.7	354-383 bp	1.0	83.9