Hotspot

trnE-UUC-trnT-GGU

LSC · 31652-32098 · 447 bp

Back to Aspidistra

Pi 0.0123

MarkerSeek score 56.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 447
variable_sites: 20
pi: 0.0123
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	544-552 bp	1.0	82.7
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	523-531 bp	1.0	82.2
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.8	58.1	520-528 bp	1.0	82.1
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	541-549 bp	1.0	81.5
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	TTGAACCGATGACTTACGCC	50.0	50.0	57.8	58.3	546-554 bp	1.0	80.4