Hotspot

trnS-GCU-trnG-UCC

LSC · 8169-9455 · 1287 bp

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Pi 0.00631
MarkerSeek score 61.00
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1287
variable_sites
58
pi
0.00631
unique_haplotype_count
27
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 GATCAGAGGGAACGGAGAGA ACGAATCGCACTTTTACCACT 55.0 42.9 57.9 58.2 1377-1435 bp 1.0 77.0
trnS-GCU-trnG-UCC_p2 AAGTGATCAGAGGGAACGGA ACGAATCGCACTTTTACCACT 50.0 42.9 58.1 58.2 1381-1439 bp 1.0 77.0
trnS-GCU-trnG-UCC_p3 ATCAGAGGGAACGGAGAGAG ACGAATCGCACTTTTACCACT 55.0 42.9 57.9 58.2 1376-1434 bp 1.0 77.0
trnS-GCU-trnG-UCC_p4 GATCAGAGGGAACGGAGAGA ACGAATCGCACTTTTACCAC 55.0 45.0 57.9 56.8 1377-1435 bp 1.0 77.0
trnS-GCU-trnG-UCC_p5 GATCAGAGGGAACGGAGAGA CGAACGAATCGCACTTTTACC 55.0 47.6 57.9 58.2 1380-1438 bp 1.0 77.0