Hotspot

rbcL

LSC · 56341-57804 · 1464 bp

Back to Asparagus

Pi 0.00904

MarkerSeek score 51.10

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1464
variable_sites: 60
pi: 0.00904
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	CAAAGGTTTCATTCGCGCAT	AAAAGGATTGGGCCGAGTTT	45.0	45.0	58.0	58.0	1604 bp	1.0	82.9
rbcL_p2	CAAAGGTTTCATTCGCGCAT	TTAGGAAAAGGATTGGGCCG	45.0	50.0	58.0	57.9	1609 bp	1.0	81.8
rbcL_p3	CAAAGGTTTCATTCGCGCAT	TGGGCCGAGTTTAATTGCAA	45.0	45.0	58.0	58.4	1596 bp	1.0	79.9
rbcL_p4	CAAAGGTTTCATTCGCGCAT	TTGGGCCGAGTTTAATTGCA	45.0	45.0	58.0	58.4	1597 bp	1.0	79.9
rbcL_p5	CAAAGGTTTCATTCGCGCAT	GAAAAGGATTGGGCCGAGTT	45.0	50.0	58.0	58.5	1605 bp	1.0	79.3