Hotspot

clpP

LSC · 71000-73093 · 2094 bp

Back to Asparagus

Pi 0.00479

MarkerSeek score 59.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2094
variable_sites: 69
pi: 0.00479
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCGCGGAAAATTAGAATTATGCC	AACTCATTCTTACGTTTCCA	39.1	35.0	57.9	52.5	2168-2203 bp	1.0	45.6
clpP_p2	CGCGGAAAATTAGAATTATGCCT	AACTCATTCTTACGTTTCCA	39.1	35.0	57.9	52.5	2167-2202 bp	1.0	45.6
clpP_p3	TCGCGGAAAATTAGAATTATGCC	AAACTCATTCTTACGTTTCCA	39.1	33.3	57.9	53.4	2169-2204 bp	1.0	45.6
clpP_p4	CGCGGAAAATTAGAATTATGCCT	AAACTCATTCTTACGTTTCCA	39.1	33.3	57.9	53.4	2168-2203 bp	1.0	45.6
clpP_p5	TCGCGGAAAATTAGAATTATGCC	ACTCATTCTTACGTTTCCAT	39.1	35.0	57.9	52.2	2167-2202 bp	0.971429	44.4