Hotspot

atpF

LSC · 11881-13300 · 1420 bp

Back to Asparagus

Pi 0.00918

MarkerSeek score 62.10

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1420
variable_sites: 78
pi: 0.00918
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CGAAGAGTTACCATTAGCGTCT	AGAACTCTGTTCTTTGTTAGTCCT	45.5	37.5	58.0	57.5	1527-1569 bp	1.0	48.1
atpF_p2	CGAAGAGTTACCATTAGCGTCT	AGAACGACTCAAGTGATACA	45.5	40.0	58.0	53.8	1550-1592 bp	1.0	48.0
atpF_p3	CGAAGAGTTACCATTAGCGTCT	AAGAACGACTCAAGTGATACA	45.5	38.1	58.0	54.7	1551-1593 bp	1.0	48.0
atpF_p4	CGAAGAGTTACCATTAGCGTCT	AGAACGACTCAAGTGATACAA	45.5	38.1	58.0	54.7	1550-1592 bp	1.0	48.0
atpF_p5	CGAAGAGTTACCATTAGCGTCT	AGAACGACTCAAGTGATACAAA	45.5	36.4	58.0	55.4	1550-1592 bp	1.0	48.0