Hotspot

accD

LSC · 58555-60030 · 1476 bp

Back to Asparagus

Pi 0.0148

MarkerSeek score 57.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1476
variable_sites: 139
pi: 0.0148
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AAACTCGGCCCAATCCTTTT	AAAGAAGCCATTGCAATCGC	45.0	45.0	58.0	58.0	2642-2855 bp	1.0	88.3
accD_p2	CCGGATTCCCCCAAAAGAAA	AAAGAAGCCATTGCAATCGC	50.0	45.0	58.1	58.0	2122-2341 bp	1.0	88.1
accD_p3	CGGCCCAATCCTTTTCCTAA	AAAGAAGCCATTGCAATCGC	50.0	45.0	57.9	58.0	2637-2850 bp	1.0	87.3
accD_p4	AAACTCGGCCCAATCCTTTT	AATCGCCGGAAATACTAGGC	45.0	50.0	58.0	57.8	2628-2841 bp	1.0	87.0
accD_p5	CCGGATTCCCCCAAAAGAAA	AATCGCCGGAAATACTAGGC	50.0	50.0	58.1	57.8	2108-2327 bp	1.0	86.9