Hotspot

ndhG-ndhI

SSC · 120359-120706 · 348 bp

Back to Asparagus

Pi 0.0192

MarkerSeek score 58.60

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 348
variable_sites: 30
pi: 0.0192
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCGTGTATTGGTCCAGGTAA	ATTCAAGAACAATTACTGATTACT	45.0	25.0	55.9	52.4	393-410 bp	1.0	50.2
ndhG-ndhI_p2	TCGTGTATTGGTCCAGGTAA	GATTCAAGAACAATTACTGATTACT	45.0	28.0	55.9	53.7	394-411 bp	1.0	50.1
ndhG-ndhI_p3	TCGTGTATTGGTCCAGGTAAA	GATTCAAGAACAATTACTGATTACT	42.9	28.0	56.7	53.7	394-411 bp	1.0	50.1
ndhG-ndhI_p4	ATCGTGTATTGGTCCAGGTA	GATTCAAGAACAATTACTGATTACT	45.0	28.0	55.6	53.7	395-412 bp	1.0	50.1
ndhG-ndhI_p5	TATCGTGTATTGGTCCAGGT	GATTCAAGAACAATTACTGATTACT	45.0	28.0	55.6	53.7	396-413 bp	1.0	50.1