Hotspot

petA-psbJ

LSC · 63855-64855 · 1001 bp

Back to Asparagus

Pi 0.00781

MarkerSeek score 60.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1001
variable_sites: 72
pi: 0.00781
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	CCGGATTGGGTTCATCTCTG	50.0	55.0	57.8	58.0	1126-1220 bp	1.0	85.1
petA-psbJ_p2	GCCTTTTGTTCTTCTTGGCG	CCGGATTGGGTTCATCTCTG	50.0	55.0	58.2	58.0	1125-1219 bp	1.0	84.5
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	TCCGGATTGGGTTCATCTCT	50.0	50.0	57.8	58.1	1127-1221 bp	1.0	84.4
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCCTGTGATCGG	50.0	55.0	57.8	58.1	1178-1272 bp	1.0	84.2
petA-psbJ_p5	GCCTTTTGTTCTTCTTGGCG	TCCGGATTGGGTTCATCTCT	50.0	50.0	58.2	58.1	1126-1220 bp	1.0	83.9