Hotspot

ndhC-trnV-UAC

LSC · 50901-52626 · 1726 bp

Back to Asparagus

Pi 0.011

MarkerSeek score 68.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1726
variable_sites: 136
pi: 0.011
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1400-1876 bp	1.0	87.5
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	AGCCCAATGCAACAAAATGC	50.0	45.0	57.9	58.5	1482-1958 bp	1.0	85.0
ndhC-trnV-UAC_p3	TCCTTCACTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	1413-1889 bp	1.0	84.8
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1399-1875 bp	1.0	84.7
ndhC-trnV-UAC_p5	CTGGTCCTTCACTAATCGGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.4	57.8	1417-1893 bp	1.0	83.7