Hotspot

trnL-UAA-trnF-GAA

LSC · 48315-48663 · 349 bp

Back to Asparagus

Pi 0.0113

MarkerSeek score 58.10

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 349
variable_sites: 33
pi: 0.0113
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	439-474 bp	1.0	82.0
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	ATTCACGTGTCAGGAACCAG	50.0	50.0	57.5	57.8	460-495 bp	1.0	80.8
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	CACGTGTCAGGAACCAGATT	50.0	50.0	57.5	57.8	457-492 bp	1.0	80.8
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGA	50.0	50.0	57.5	57.6	401-436 bp	1.0	79.4
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	436-471 bp	1.0	78.8