Hotspot

psaA-ycf3

LSC · 42188-42827 · 640 bp

Back to Asparagus

Pi 0.00827

MarkerSeek score 65.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 640
variable_sites: 31
pi: 0.00827
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGATGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.0	57.8	736-807 bp	1.0	85.1
psaA-ycf3_p2	GCCCATTCCTCGAAAGATGT	TGAAGATCACGAGGCGTTTC	50.0	50.0	58.0	58.3	735-806 bp	1.0	84.1
psaA-ycf3_p3	GCCCATTCCTCGAAAGATGT	TCACGAGGCGTTTCGAATAA	50.0	45.0	58.0	57.6	729-800 bp	1.0	83.6
psaA-ycf3_p4	GCCCATTCCTCGAAAGATGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.0	58.2	740-811 bp	0.971429	83.3
psaA-ycf3_p5	GCCCATTCCTCGAAAGATGT	GCTATAGCGCTTACTCCAGG	50.0	55.0	58.0	57.6	781-852 bp	1.0	83.1