Hotspot

rpoB-trnC-GCA

LSC · 26787-28015 · 1229 bp

Back to Asparagus

Pi 0.00744

MarkerSeek score 60.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1229
variable_sites: 63
pi: 0.00744
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AAATTCTTCTGCCAAGCCCT	ATCTTTTGTTGCTCAGGCGA	45.0	45.0	57.7	58.1	1413-1463 bp	1.0	82.8
rpoB-trnC-GCA_p2	CTTCTGCCAAGCCCTGATTA	ATCTTTTGTTGCTCAGGCGA	50.0	45.0	57.6	58.1	1408-1458 bp	1.0	81.9
rpoB-trnC-GCA_p3	AATTCTTCTGCCAAGCCCTG	ATCTTTTGTTGCTCAGGCGA	50.0	45.0	58.4	58.1	1412-1462 bp	1.0	81.7
rpoB-trnC-GCA_p4	TCCCTCATTTCCATTCCGGA	ATCTTTTGTTGCTCAGGCGA	50.0	45.0	58.4	58.1	1332-1382 bp	0.942857	79.9
rpoB-trnC-GCA_p5	TTCCCTCATTTCCATTCCGG	ATCTTTTGTTGCTCAGGCGA	50.0	45.0	57.6	58.1	1333-1383 bp	0.942857	79.7