Hotspot

ycf1

SSC · 131751-137543 · 5793 bp

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Pi 0.0142

MarkerSeek score 53.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5793
variable_sites: 78
pi: 0.0142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCTCCGCAATGATGGATTC	AACCAACAGCGATTTCACCT	50.0	45.0	58.0	58.0	1114 bp	0.5	59.6
ycf1_p2	ACCTCCGCAATGATGGATTC	ACCAACAGCGATTTCACCTT	50.0	45.0	58.0	58.0	1113 bp	0.5	59.6
ycf1_p3	GAACCTCCGCAATGATGGAT	AACCAACAGCGATTTCACCT	50.0	45.0	58.0	58.0	1116 bp	0.5	59.6
ycf1_p4	GAACCTCCGCAATGATGGAT	ACCAACAGCGATTTCACCTT	50.0	45.0	58.0	58.0	1115 bp	0.5	59.6
ycf1_p5	AGAGTGCGCTTTTCCTTGAA	ACCAACAGCGATTTCACCTT	45.0	45.0	58.0	58.0	991 bp	0.5	59.6